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Mr Gary Duncan

Mr Gary Duncan
Mr Gary Duncan
Mr Gary Duncan

Research Technician

About
Email Address
gary.duncan@abdn.ac.uk
Telephone Number
+44 (0)1224 438669
School/Department
School of Medicine, Medical Sciences and Nutrition

Biography

Gary has over 25 years of Mass Spectrometry experience.  He started work at what was then the Rowett Research Institute in 1986, and after six weeks training in analytical techniques, started working in the Gas Isotope Ratio Mass Spectrometry (GIRMS) laboratory assisting Dr Brian McGaw and Mr Eric Milne. As interest in this technique grew, the lab eventually had four mass spectrometers dedicated to analysing the stable isotopes of Carbon, Hydrogen and Oxygen from a variety of biological sources, supporting research into energy expenditure and protein synthesis. The research programmes of the Rowett then required Liquid Chromatography Mass Spectrometry (LC/MS) and a MAT 900 double-sector LC-MS/MS system was purchased in 1998, along with a LaserMAT MALDI mass spectrometer. Initially his role was to develop techniques for sample analysis on the MALDI, but soon took over the role of managing the LC/MS mass facility. This coincided with a ‘take-off’ in the popularity of using LCMSMS in sample analysis, firstly in the growth of identifying proteins separated on 2D gels and more recently the targeted quantitation analysis of small molecules. The main areas of analysis are in the quantification of Polyphenols relating to dietary consumption and human health and the quantification of Mycotoxins in foods and crops, in particular, the detoxification of Deoxynivalenol. The LCMSMS facility now has four mass spectrometers, with access to an ion trap to support work in these areas of research.

Other Areas of interest include:

  • Vitamin D3
  • Methylamines
  • Anthocyanins
  • Nicotinamides
  • Carboxymethyl Lysines
  • Antibiotics

Latest Publications

View My Publications

Publications

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  • Nutritional B vitamin deficiency alters the expression of key proteins associated with vascular smooth muscle cell proliferation and migration in the aorta of atherosclerotic apolipoprotein E null mice

    Duthie, S. J., Beattie, J. H., Gordon, M., Pirie, L. P., Nicol, F., Reid, M. D., Duncan, G. J., Cantlay, L., Horgan, G., McNeil, C. J.
    Genes & Nutrition, vol. 10, no. 1, 446
    Contributions to Journals: Articles

  • Role of Pathogen-Derived Cell Wall Carbohydrates and Prostaglandin E2 in Immune Response and Suppression of Fish Immunity by the Oomycete Saprolegnia parasitica

    Belmonte, R., Wang, T., Duncan, G. J., Skaar, I., Mélida, H., Bulone, V., van West, P., Secombes, C. J.
    Infection and Immunity, vol. 82, no. 11, pp. 4518-4529
    Contributions to Journals: Articles

  • Annual variation of dietary deoxynivalenol exposure during years of different Fusarium prevalence: a pilot biomonitoring study

    Gratz, S. W., Richardson, A. J., Duncan, G., Holtrop, G.
    Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, vol. 31, no. 9, pp. 1579-1585
    Contributions to Journals: Articles

  • Bound phytophenols from ready-to-eat cereals: comparison with other plant-based foods

    Neacsu, M., McMonagle, J., Fletcher, R. J., Scobbie, L., Duncan, G. J., Cantlay, L., de Roos, B., Duthie, G. G., Russell, W. R.
    Food Chemistry, vol. 141, no. 3, pp. 2880-2886
    Contributions to Journals: Articles

  • Microbial metabolism and intestinal absorption of the mycotoxin deoxynivalenol and its metabolites in humans

    Gratz, S., Duncan, G., Richardson, A. J.
    Annals of Nutrition & Metabolism, vol. 63, no. Suppl 1, pp. 1806
    Contributions to Journals: Abstracts

  • A methyl-deficient diet fed to rats during the pre- and peri-conception periods of development modifies the hepatic proteome in the adult offspring

    Maloney, C. A., Hay, S. M., Reid, M. D., Duncan, G., Nicol, F., Sinclair, K. D., Rees, W. D.
    Genes & Nutrition, vol. 8, no. 2, pp. 181-190
    Contributions to Journals: Articles

  • Human fecal microbiota metabolize deoxynivalenol and deoxynivalenol-3-glucoside and may be responsible for urinary de-epoxy deoxynivalenol

    Gratz, S. W., Duncan, G., Richardson, A. J.
    Applied and Environmental Microbiology, vol. 79, no. 6, pp. 1821-1825
    Contributions to Journals: Articles

  • Major phenylpropanoid-derived metabolites in the human gut can arise from microbial fermentation of protein

    Russell, W. R., Duncan, S. H., Scobbie, L., Duncan, G., Cantlay, L., Calder, A. G., Anderson, S. E., Flint, H. J.
    Molecular Nutrition & Food Research, vol. 57, no. 3, pp. 523-535
    Contributions to Journals: Articles

  • Inter-organ proteomic analysis reveals insights into the molecular mechanisms underlying the anti-diabetic effects of cis-9, trans-11-conjugated linoleic acid in ob/ob mice

    Rungapamestry, V., McMonagle, J., Reynolds, C., Rucklidge, G., Reid, M., Duncan, G., Ross, K., Horgan, G., Toomey, S., Moloney, A. P., de Roos, B., Roche, H. M.
    Proteomics, vol. 12, no. 3, pp. 461-476
    Contributions to Journals: Articles

  • Anti-platelet effects of olive oil extract: in vitro functional and proteomic studies

    de Roos, B., Zhang, X., Rodriguez Gutierrez, G., Wood, S., Rucklidge, G. J., Reid, M. D., Duncan, G. J., Cantlay, L. L., Duthie, G. G., O'Kennedy, N.
    European Journal of Nutrition, vol. 50, no. 7, pp. 553-562
    Contributions to Journals: Articles

  • High-protein, reduced-carbohydrate weight-loss diets promote metabolite profiles likely to be detrimental to colonic health

    Russell, W. R., Gratz, S. W., Duncan, S. H., Holtrop, G., Ince, J., Scobbie, L., Duncan, G., Johnstone, A. M., Lobley, G. E., Wallace, R. J., Duthie, G. G., Flint, H. J.
    The American Journal of Clinical Nutrition, vol. 93, no. 5, pp. 1062-1072
    Contributions to Journals: Articles

  • Impact of Obesity and Leptin on Protein Expression Profiles in Mouse Colon

    Padidar, S., Farquharson, A. J., Williams, L. M., Hoggard, N., Reid, M. D., Duncan, G. J., Drew, J. E.
    Digestive Diseases and Sciences, vol. 56, no. 4, pp. 1028-1036
    Contributions to Journals: Articles

  • Blood folate status and expression of proteins involved in immune function, inflammation, and coagulation: biochemical and proteomic changes in the plasma of humans in response to long-term synthetic folic acid supplementation

    Duthie, S. J., Horgan, G., De Roos, B., Rucklidge, G., Reid, M., Duncan, G., Pirie, L., Basten, G. P., Powers, H. J.
    Journal of Proteome Research, vol. 9, no. 4, pp. 1941-1950
    Contributions to Journals: Articles

  • Intervention with fish oil, but not with docosahexaenoic acid, results in lower levels of hepatic soluble epoxide hydrolase with time in apoE knockout mice

    Mavrommatis, Y., Ross, K., Rucklidge, G., Reid, M., Duncan, G. J., Gordon, M. J., Thies, F., Sneddon, A. A., De Roos, B.
    British Journal of Nutrition, vol. 103, no. 1, pp. 16-24
    Contributions to Journals: Articles

  • Maternal diets deficient in folic acid and related methyl donors modify mechanisms associated with lipid metabolism in the fetal liver of the rat

    McNeil, C. J., Hay, S. M., Rucklidge, G. J., Reid, M. D., Duncan, G. J., Rees, W. D.
    British Journal of Nutrition, vol. 102, no. 10, pp. 1445-1452
    Contributions to Journals: Articles

  • Balancing Inflammatory, Lipid, and Xenobiotic Signaling Pathways by VSL#3, a Biotherapeutic Agent, in the Treatment of Inflammatory Bowel Disease

    Reiff, C., Delday, M., Rucklidge, G., Reid, M., Duncan, G., Wohlgemuth, S., Hoermannsperger, G., Loh, G., Blaut, M., Collie-Duguid, E., Haller, D., Kelly, D.
    Inflammatory Bowel Diseases, vol. 15, no. 11, pp. 1721-1736
    Contributions to Journals: Articles

  • Gene and protein expression profiles in the foetal liver of the pregnant rat fed a low protein diet

    McNeil, C. J., Hay, S. M., Rucklidge, G. J., Reid, M. D., Duncan, G. J., Rees, W. D.
    Genes & Nutrition, vol. 4, no. 3, pp. 189-194
    Contributions to Journals: Articles

  • Phenolic acid content of fruits commonly consumed and locally produced in Scotland

    Russell, W. R., Labat, A., Scobbie, L., Duncan, G. J., Duthie, G. G.
    Food Chemistry, vol. 115, no. 1, pp. 100-104
    Contributions to Journals: Articles

  • Attenuation of inflammation and cellular stress-related pathways maintains insulin sensitivity in obese type I interleukin-1 receptor knockout mice on a high-fat diet

    de Roos, B., Rungapamestry, V., Ross, K., Rucklidge, G., Reid, M., Duncan, G., Horgan, G., Toomey, S., Browne, J., Loscher, C. E., Mills, K. H. G., Roche, H. M.
    Proteomics, vol. 9, no. 12, pp. 3244-3256
    Contributions to Journals: Articles

  • The response of human colonocytes to folate deficiency in vitro: functional and proteomic analyses

    Duthie, S. J., Mavrommatis, Y., Rucklidge, G., Reid, M., Duncan, G., Moyer, M. P., Pirie, L. P., Bestwick, C. S.
    Journal of Proteome Research, vol. 7, no. 8, pp. 3254-3266
    Contributions to Journals: Articles

  • Aorta protein networks in marginal and acute zinc deficiency

    Beattie, J. H., Gordon, M. J., Rucklidge, G. J., Reid, M. D., Duncan, G. J., Horgan, G. W., Cho, Y., Kwun, I.
    Proteomics, vol. 8, no. 10, pp. 2126-2135
    Contributions to Journals: Articles

  • Identification of potential serum biomarkers of inflammation and lipid modulation that are altered by fish oil supplementation in healthy volunteers

    De Roos, B., Geelen, A., Ross, K., Rucklidge, G., Reid, M., Duncan, G., Caslake, M., Horgan, G., Brouwer, I. A.
    Proteomics, vol. 8, no. 10, pp. 1965-1974
    Contributions to Journals: Articles

  • Disruption of lipid metabolism in the liver of the pregnant rat fed folate-deficient and methyl donor-deficient diets

    McNeil, C. J., Hay, S., Rucklidge, G. J., Reid, M. D., Duncan, G. J., Maloney, C. A., Rees, W.
    British Journal of Nutrition, vol. 99, no. 2, pp. 262-271
    Contributions to Journals: Articles

  • Profiling of mitochondrial associated proteins from rat colon

    Padidar, S., Bestwick, C., King, T. P., Rucklidge, G. J., Duncan, G. J., Reid, M. D., Drew, J.
    Journal of Cellular Biochemistry, vol. 103, no. 1, pp. 78-97
    Contributions to Journals: Articles

  • The effects of fish oil and DHA on soluble epoxide hydrolase in apoE-/- mice

    Mavrommatis, Y. I., Thies, F., Ross, K., Gordon, M. J., McLeod, E., Sneddon, A. A., Rucklidge, G. J., Reid, M. D., Duncan, G. J., Mayer, C., Horgan, G., Arthur, J., De Roos, B.
    Proceedings of the Nutrition Society, vol. 66, no. Supplement OCA-B , 103A
    Contributions to Journals: Abstracts
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