We have a dedicated preparation area for setting up qPCR experiments. All the equipment required is provided and consumables such as tips are included in plate charges (see
charges for more details).
Instruments
LightCycler 480 II (3 instruments)
96 & 384 well blocks available
1h run time
Wide range of assay formats supported:
UPL
Hydrolysis probes (TaqMan)
Simple probes
SYBR green I
HybProbes
MIQE compliant data analysis
Multiplexing - up to 5 colours
Assays available:
Gene expression (relative and absolute quantification)
Gene scanning
Endpoint genotyping
Melt curve genotyping
HRM
StepOne Plus
Dedicated 96 well block
Standard and fast modes
Touchscreen or PC based controls
High performance qPCR and HRM
Supports of full range of TaqMan assays
Analysis software available to download
3 colour multiplexing (ROX normalisation)
Veriflex block - temperature control, ability to set gradients
Assays available:
SNP genotyping
Gene expression (absolute and relative quantification)
