The Oxford Nanopore MinION and MinION Mk1C devices provide long read sequencing capacity of up to ~20 Gb output, dependent upon DNA quality and flow cell choice. Read length is determined by DNA quality, extraction and preparation methods, but is not limited by the hardware. The Mk1c provides localised compute power, with an in-built GPU, allowing for real-time base calling and portability. Long reads and ultra-long reads can be obtained from amplified DNA, RNA/ cDNA or amplicons for applications such as improving/ closing genome assemblies, structural variant analysis, phasing, splice variant analysis, fusion transcripts and real-time metagenomics. Direct DNA or RNA sequencing is also possible and allows determination of base modifications for epigenetic applications such as methylome analysis or analysis of the epitranscriptome. We have recently established an amplicon pipeline for rapid strain level resolution of pathogens.
